Micropropagation of Vaccinium corymbosum in Murashige and Skoog medium (1962), supplemented with 6-benzylaminopurine

Abstract

Vaccinium corymbosum"cranberry" is a fruit that belongs to the family of Ericaceae that has a high nutritional and medicinal value. Plant biotechnology uses micropropagation as a tool that allows obtaining numerous seedlings in a smaller proportion of time, for this it is necessary to standardize the concentration of culture media and growth regulators. Given the need for greater knowledge in this area, it was proposed as a research objective to determine the optimal concentration of 6-benzylaminopurine (BAP) between Murashige and Skoog, 1962. The experimental phase was developed in the Biotechnology Laboratory of the Institute of Potato and Andean Crops of the National University of Trujillo. Where internodes from previously treated and conditioned mother plants were used, to be established in Murashige and Skoog culture medium with five BAPtreatments: T1 (0 ppm), T2 (8 ppm), T3 (10 ppm), T4 (12 ppm) and T5 (15 ppm).Acompletely randomized experimental design was used, applying ANOVAand Tukey. Significant differences were found between treatments, for the variables stem length (0.0403 <p) and number of leaves (0.0132 <p) in the in vitro establishment. It is concluded that the BAPat the concentration of 15 ppm is the most optimal for the in vitro micropropagation of V. corymbosum"cranberry".